pturbo-GFP-B 熒光表達載體 BioVector NTCC質粒載體菌種細胞基因保藏中心

Map:

Vector type bacterial expression vector
Reporter TurboGFP
Reporter codon usage mammalian
Promoter for TurboGFP T5 promoter/lac operator
Host cells prokaryotic
Selection ampicillin
Replication ColE1 ori
Use Source of the TurboGFP coding sequence; TurboGFP
expression in bacterial cells
Vector description
pTurboGFP-B is a prokaryotic expression vector encoding green fluorescent protein TurboGFP. Reporter codon
usage is optimized for high expression in mammalian cells (humanized) [Haas et al. 1996].
The vector is primarily intended as a source of TurboGFP coding sequence. Flanking restriction sites are
convenient for excision of TurboGFP sequence and its further insertion into other expression vectors of choice.
Alternatively, TurboGFP coding sequence can be amplified by PCR.
Note: The plasmid DNA was isolated from dam+-methylated E.coli. Therefore some restriction sites are blocked by methylation. If you wish to
digest the vector using such sites you will need to transform the vector into a dam- host and make fresh DNA.
The vector can be also used for TurboGFP expression in prokaryotes under the control of T5 promoter/lac operator.
The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation
and selection in E. coli.
Location of features
T5 promoter/lac operator element: 7-87
T5 transcription start: 61
TurboGFP coding sequence: 132-827
Lambda t0 transcriptional termination region: 848-942
rrnB T1 transcriptional termination region: 1704-1802
ColE1 origin of replication: 2278
beta-lactamase coding sequence: 3896-3036
【Supplier來源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website網址】 http://www.biovector.net