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載體基本信息
| 出品公司: | Addgene |
|---|---|
| 載體名稱: | pRSV-rev |
| 質粒類型: | 慢病毒系統；包裝載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 啟動子: | RSV U3 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 4174bp |
| 5' 測序引物及序列: | pREP fwd: GCTCGATACAATAAACGCC |
| 3' 測序引物及序列: | -- |
| 載體標簽: | -- |
| 載體抗性: | 氨芐青霉素（Ampicillian） |
| 篩選標記: | -- |
| 備注: | 第三代慢病毒系統包裝載體。 |
| 產品目錄號: | 12253 |
| 穩定性: | -- |
| 組成型: | -- |
| 病毒/非病毒: | 慢病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system.載體序列
LOCUS pRSV-Rev 4174 bp DNA SYN
DEFINITION pRSV-Rev
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4174
/organism="pRSV-Rev"
/mol_type="other DNA"
promoter 143..172
/label="lac_promoter"
misc_feature 186..208
/label="M13_pUC_rev_primer"
promoter 207..225
/label="M13_reverse_primer"
misc_feature 579..597
/label="pREP_fwd_primer"
misc_feature 638..662
/label="LNCX_primer"
misc_feature 978..1007
/label="HIV_Rev_NES"
promoter complement(1190..1206)
/label="M13_forward20_primer"
rep_origin 1549..1855
/label="f1_origin"
misc_feature 2123..2145
/label="pGEX_3_primer"
promoter 2304..2332
/label="AmpR_promoter"
gene 2374..3234
/label="Ampicillin"
/gene="Ampicillin"
CDS 2374..3234
/label="ORF frame 1"
rep_origin 3389..4008
/label="pBR322_origin"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgatg
241 tacgggccag atatacgcgt atctgagggg actaggtgtg tttaggcgaa aagcggggct
301 tcggttgtac gcggttagga gtcccctcag gattagtagt ttcgcttttg catagggagg
361 gggaaatgta gtcttatgca atacacttgt agtcttgcaa catggtaacg atgagttagc
421 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
481 acgatcgtgc cttattagga aggcaacaga caggtctgac atggattgga cgaaccactg
541 aattccgcat tgcagagata attgtattta agtgcctagc tcgatacaat aaacgccatt
601 tgaccattca ccacattggt gtgcacctcc aagctcgagc tcgtttagtg aaccgtcaga
661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
721 gcctcccctc gaagctagtc gattaggcat ctcctatggc aggaagaagc ggagacagcg
781 acgaagacct cctcaaggca gtcagactca tcaagtttct ctatcaaagc aacccacctc
841 ccaatcccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
901 agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg
961 agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt
1021 gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa
1081 tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac agctatagca
1141 gtagctgagg ggacagatag ggttatagaa gtagtacaag aagcttggca ctggccgtcg
1201 ttttacatga tctgagcctg ggagatctct ggctaactag ggaacccact gcttaagcct
1261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
1321 aactagagat caggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
1381 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
1441 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
1501 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
1561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
1621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
1681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
1741 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
1801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
1861 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
1921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
1981 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
2041 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
2101 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
2161 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
2221 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
2281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
2341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
2401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
2461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
2521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
2581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
2641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
2701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
2761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
2821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
2881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
2941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
3001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
3061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
3121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
3181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
3241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
3301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
3361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
3421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
3481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
3541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
3601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
3661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
3721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
3781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
3841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
3901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
3961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
4141 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
載體質粒圖譜pdf版和相關資料下載
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