pNTAP-Mef2a哺乳動物細胞TAP標簽表達質粒載體CBP和SBP標簽 BioVector NTCC質粒載體菌種細胞基因保藏中心
pNTAP-Mef2a and pCMV-Tag2-Mef2c
Expression Control Vectors
The pNTAP-Mef2a control vector contains the MEF2a ORF tagged at the
N-terminus with SBP and CBP tags. The pCMV-Tag2-Mef2c control vector
contains the MEF2c ORF tagged at the N-terminus with the FLAG tag.
(See Figure 5.) When co-transfected, expressed MEF2 proteins from each
vector interact and are co-purified via tandem affinity purification using the
streptavidin and calmodulin resins. The purified MEF2c protein is detected
by Western blotting using an antibody to the FLAG peptide, indicating that
it interacted and co-purified with its partner, MEF2a. The control proteins
can be further characterized by mass spectrometry analysis, validating
interaction.
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