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lacY E.coli mutant大腸桿菌基因缺失菌株-BioVector NTCC保藏中心
Gene Symbol突變基因名稱：lacY
Mnemonic:lactose
Synonyms別稱：
Gene product基因產物：galactoside permease (M protein)
Selectable Phenotype篩選表型：form white colonies on MacConkey lactose plates
Culture Condition培養條件：DYT broth containing 1.6 % Bacto tryptone (Difco), 1.0 % yeast extract (Difco), and 0.5 % NaCl
Other Phenotypes其他表型：•form blue colonies on MacConkey-lactose plate containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (xgal, 0.04 g/li with 4 g/l of glucose as a carbon source) and ispropyl-thio-beta-D-galactoside (IPTG, 0.048 g/l) (WT: dark purple, lacZ-lacY-: white)
•resistant to o-nitrophenyl-beta-D-galactopyranoside (2 mM) (J. Bacteriol. 1973. 114: 882-884 )
Notes：
Reference參考文獻：Hobson, A. C. et al.
J. Bacteriol. 1977. 131. 830-833
Supplier：
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net
Gene Symbol突變基因名稱：lacY
Mnemonic:lactose
Synonyms別稱：
Gene product基因產物：galactoside permease (M protein)
Selectable Phenotype篩選表型：form white colonies on MacConkey lactose plates
Culture Condition培養條件：DYT broth containing 1.6 % Bacto tryptone (Difco), 1.0 % yeast extract (Difco), and 0.5 % NaCl
Other Phenotypes其他表型：•form blue colonies on MacConkey-lactose plate containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (xgal, 0.04 g/li with 4 g/l of glucose as a carbon source) and ispropyl-thio-beta-D-galactoside (IPTG, 0.048 g/l) (WT: dark purple, lacZ-lacY-: white)
•resistant to o-nitrophenyl-beta-D-galactopyranoside (2 mM) (J. Bacteriol. 1973. 114: 882-884 )
Notes：
Reference參考文獻：Hobson, A. C. et al.
J. Bacteriol. 1977. 131. 830-833
Supplier：
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net
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