pBI-GL四環(huán)素誘導(dǎo)表達(dá)Luc熒光報(bào)告載體質(zhì)粒 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
The pBI-L Tet Vector is a response plasmid that can be used to express a gene of interest
and luciferase from a bidirectional tet-responsive promoter (Pbi-1; 1) in Clontech’s Tet-On and
Tet-Off Gene Expression Systems and Cell Lines (2). The Tet Expression Systems and Cell Lines
give researchers ready access to the tetracycline-regulated expression systems described by
Gossen & Bujard (3; Tet-Off) and Gossen et al. (4; Tet-On). The pBI-L Tet Vector contains the
bidirectional promoter Pbi-1 which is responsive to the tTA and rtTA regulatory proteins in the
Tet-Off and Tet-On systems, respectively. Pbi-1 contains the Tet-responsive element (TRE), which
consists of seven copies of the 42-bp tet operator sequence (tetO). The TRE element is between
two minimal CMV promoters (PminCMV), which lack the enhancer that is part of the complete
CMV promoter. Consequently, Pbi-1 is silent in the absence of binding of TetR or rTetR to the
tetO sequences. PminCMV-1 controls the expression of the gene of interest; and PminCMV-2 controls
the expression of luciferase. Therefore, the expression of a gene of interest for which there is
no convenient assay may be monitored indirectly via the luciferase reporter function. Note that
the cloned insert must have an initiation codon. In some cases, addition of a Kozak consensus
ribosome binding site (5) may improve expression levels; however, many cDNAs have been
efficiently expressed in Tet systems without the addition of a Kozak sequence.
【Supplier來源】BioVector NTCC Inc.
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