產(chǎn)品名稱SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody, Rabbit MAb
經(jīng)驗(yàn)證的應(yīng)用WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特異性2019-nCoV CoV Nucleocapsid
Has cross-reactivity in ELISA with SARS-CoV Nucleoprotein / NP Protein.
來源Monoclonal Rabbit IgG Clone #019
純化Protein A & Antigen Affinity
緩沖液0.2 μm filtered solution in PBS
偶聯(lián)物Unconjugated
狀態(tài)Liquid
運(yùn)輸方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
儲存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody, Rabbit MAb (Rabbit Monoclonal 抗體) 經(jīng)驗(yàn)證的應(yīng)用
應(yīng)用 推薦稀釋比/用量
WB 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody, Rabbit MAb (Rabbit Monoclonal 抗體) 圖片
Anti-2019-nCoV NP rabbit monoclonal antibody at 1:1000 dilution.

Sample: 2019-nCoV NP Protein

Lane A: 30ng

Lane B: 10ng

Lane C: 5ng

Lane D: 2ng

Lane E: 1ng

Lane F: 0.5ng

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody, Rabbit MAb: 別稱
Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-NCP-CoV Nucleocapsid Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-np Antibody; Anti-nucleocapsid Antibody; Anti-Nucleoprotein Antibody
CoV Nucleocapsid 背景信息
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
研究領(lǐng)域
Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen
參考文獻(xiàn)
1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
Li W. et al., 2005, Science. 309 (5742): 1864-8.

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