產品名稱
SARS-CoV Spike Antibody, Rabbit PAb, Antigen Affinity Purified
經驗證的應用
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS CoV spike
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Spike S1 (BioVector40591-V08H)
SARS-CoV-2 (2019-nCoV) Spike RBD (BioVector40592-V05H)
免疫原
Recombinant SARS-CoV Spike S1 Subunit Protein (BioVector40150-V08B1)
制備方法
Produced in rabbits immunized with purified, recombinant SARS-CoV Spike S1 Subunit Protein (BioVector40150-V08B1; AAX16192.1; Met1-Arg667). The specific IgG was purified by SARS-CoV Spike S1 Subunit affinity chromatography.
來源
Polyclonal Rabbit IgG
純化
Protein A & Antigen Affinity
緩沖液
0.2 μm filtered solution in PBS
偶聯物
Unconjugated
狀態
Liquid
運輸方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
儲存條件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
SARS-CoV Spike Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗體) 經驗證的應用
應用 推薦稀釋比/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.
SARS-CoV Spike Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗體) 圖片
Anti SARS-CoV S1 rabbit polyclonal antibody at 1:2000 dilution.
Lane A: SARS-CoV Spike S1 (BioVector40150-V08B1) (5ng)
Lane B: SARS-CoV-2 (2019-nCoV) Spike S1 (BioVector40591-V08H) (30ng)
Lane C: SARS-CoV-2 (2019-nCoV) Spike RBD (BioVector40592-V05H) (30ng)
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
SARS-CoV Spike Antibody, Rabbit PAb, Antigen Affinity Purified: 別稱
Anti-coronavirus s1 Antibody; Anti-coronavirus s2 Antibody; Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus s1 Antibody; Anti-novel coronavirus s2 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-s2 Antibody; Anti-Spike RBD Antibody
CoV spike 背景信息
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
研究領域
? Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen
參考文獻
? Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
? Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
? Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.



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