產品名稱
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified
經驗證的應用
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
應用說明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS CoV Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Protein (BioVector 40588-V08B).
免疫原
Recombinant SARS-CoV Nucleoprotein / NP Protein (BioVector40143-V08B)
制備方法
Produced in rabbits immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (BioVector40143-V08B; NP_828858.1; Met1-Ala422). SARS-CoV Nucleoprotein / NP specific IgG was purified by SARS-CoV Nucleoprotein / NP affinity chromatography.
來源
Polyclonal Rabbit IgG
純化
Protein A & Antigen Affinity
緩沖液
0.2 μm filtered solution in PBS
偶聯物
Unconjugated
狀態
Liquid
運輸方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
儲存條件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗體) 經驗證的應用
應用 推薦稀釋比/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗體) 圖片
Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution.
Lane A: SARS-CoV NP Protein (BioVector40143-V08B) (30ng)
Lane B: SARS-CoV NP Protein (BioVector40143-V08B) (5ng)
Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (BioVector40143-V08B) (30ng)
Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (BioVector40143-V08B) (5ng)
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified: 別稱
Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody
CoV Nucleocapsid 背景信息
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
研究領域
? Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen
參考文獻
1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
3. Li W. et al., 2005, Science. 309 (5742): 1864-8.



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