BL-3 Bos taurus, cow B lymphocyte cell line，CRL-8037 BioVector NTCC質粒載體菌種細胞基因保藏中心

Organism  Bos taurus, cow
Cell Type  B lymphocyte
Product Format  frozen
Morphology  lymphoblast
Culture Properties  suspension
Biosafety Level  2 Cells contain Bovine Viral Diarrhea Virus (BVDV)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease  lymphosarcoma; leukemia
Age  3 months
Gender  male
Strain  Hereford
Applications  
The cells do not produce bovine leukemia virus.
They are of possible use in making vaccines against bovine leukemia virus.
Clinical Data  
male
Comments  
The cells do not produce bovine leukemia virus.
They are of possible use in making vaccines against bovine leukemia virus.
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium  The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing  
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation  
culture medium, 90%; DMSO, 10%
Culture Conditions  
Temperature: 37.0°C
U.S. Patent Number  
4,323,555

Supplier來源:BioVector NTCC Inc.
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Website網址: http://www.biovector.net