KG-1a人急性骨髓白血病細胞KG1a cell line

       Cell Line Origin

       Human Caucasian bone marrow acute myelogenous leukaemia        

       Cell Line Description

       Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.        

       DNA Profile

       STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19        

       Culture Medium

       IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).        

       Subculture Routine

       Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO₂; 37°C.        

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