WEA枯草芽孢桿菌食品級表達菌株Bacillus subtilis strain

For expressing thegene of interest under food grade conditions,two B. subtilisstrains are available; TEA  and  WEA.  Both  strainscontain  the  toxin  expression  cassette ydcEunder control   of   the   xylose-inducible   promoter   Pxyl,   whereas   the   former ydcDEoperon (containing the toxin and antitoxin gene)is deleted.The strain B. subtilisTEA is based on B.  subtilis168  Marburg  and  is  recommended  for  intracellular  protein  expression  and pathway engineering. B. subtilisWEA originated from the eightfold extracellular protease-deficient  strain B.  subtilisWB800N  and  is  particularly  constructed  for  secretory  protein production.Both strains  are  suitable  hosts  for boththefood  grade  low copy  expression vector pTTB1and the high copy expression vector pTTB2.TEA: trpC2ydcDE::Pxyl-ycdEWEA:nprE  aprE  epr  bpr  mpr::ble  nprB::bsrΔvpr  wprA::hyg  cm::neoydcDE::Pxyl-ycdE; NeoR

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