Jurkat-Lat cell line人外周血白血病T細胞系

Alias: J-Lat cell line

Description:  This is a Jurkat-based cell line containing a full-length integrated HIV-1 genome that expresses GFP upon activation. The genome generates incomplete virions due to a frameshift in env. Special Characteristics:  Jurkat cells were infected with the packaged retroviral construct HIV-R7/E-/GFP, which is full length HIV-1 genome with a non-functional Env due to a frameshift, and GFP in place of the Nef gene. Full-length constructs secrete incomplete viral particles (capsids). The cells express low to undetectable levels of GFP under basal conditions. Suited to study HIV latency and reactivation. The clones in this series are: 6.3 , 8.4 , 9.2 , 10.6, and 15.4. Please see Table I in the reference publication for differences between these clones in GFP and p24 expression upon stimulation with TNF-α Cell Type:  Jurkat - T lymphocyte cell line Freeze Medium:  FBS, 90%; DMSO, 10%. Growth Characteristics:  No special requirements, split 1:3 at 1x106 cells/ml. Cells grow in suspension, usually singly but some clumping has been noted. Morphology:  Small, spherical cells in suspension. Morphology usually does not vary. Propagation Medium:  RPMI 1640, 90%; FBS, 10%; supplemented with penicillin G (100 U/ml), streptomycin (100 μg/ml), L-glutamine (2 mM, 0.3 mg/ml). Sterility:  Negative for bacteria, mycoplasma, and fungi. References:  Jordan, A., Bisgrove, D., & Verdin, E. (2003). HIV reproducibly establishes a latent infection after acute infection of T cells in vitro. EMBO J, 22(8), 1868-1877. doi:10.1093/emboj/cdg188 PUBMED

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