BL21-Gold，BL21-Gold（DE3），BL21- Gold（DE3）pLysS大腸桿菌表達宿主菌/感受態細胞

BL21-Gold competent cells, BL21-Gold(DE3) competent cells, and BL21-
Gold(DE3)pLysS competent cells are improved versions of BL21 competent
cells.3
These high-performance competent cells provide increased
transformation efficiency and produce high-quality miniprep DNA.
These BL21-Gold-derived expression strains are ideal for performing
protein expression studies that utilize the T7 RNA polymerase promoter to
direct high-level expression. Derived from Escherichia coli B, these
expression strains naturally lack the Lon protease, which can degrade
recombinant proteins. In addition, these strains are engineered to be
deficient for a second protease, the OmpT protein.
These BL21-Gold-derived expression strains incorporate major
improvements over the original BL21 strain. The BL21-Gold strains feature
the Hte phenotype present in the highest efficiency Agilent competent cell
strain, XL10-Gold.4
The presence of the Hte phenotype increases the
transformation efficiency of the BL21-Gold cells to >1 × 108 cfu/μg of
pUC18 DNA. In addition, the gene that encodes endonuclease I (endA),
which rapidly degrades plasmid DNA isolated by most miniprep procedures,
is inactivated. These two improvements allow direct cloning of many
protein expression constructs.
All three of the BL21-Gold-derived expression strains are resistant to
tetracycline. In addition, the BL21-Gold(DE3)pLysS strain is resistant to
chloramphenicol.
Host Strains and Genotypes
Host strain Reference Genotype
BL21-Gold straina
1 E. coli B F–
ompT hsdS(rB–
mB–
) dcm+ Tetr gal endA Hte
BL21-Gold(DE3) straina
1 E. coli B F–
ompT hsdS(rB–
mB–
) dcm+ Tetr gal λ(DE3) endA Hte
BL21-Gold(DE3)pLysS straina
1 E. coli B F–
ompT hsdS(rB–
mB–
) dcm+ Tetr gal λ(DE3) endA Hte [pLysS Camr
]

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