BaF3_EML4-ALK-G1202R_Cell_Line激酶靶點穩轉細胞株-BioVector NTCC典型培養物保藏中心

Cell line name:Ba/F3 EML4-ALK-G1202R Cell LineGene ID/Accession #: Host cell lineMouse Ba/F3 cell line Cell type: Pro-B cellsDescription: Stable Ba/F3 clone expressing exogenous EML4-ALK fusion gene with G1202R point mutation in the ALK part.Quantity:One vial of frozen cells (5X106 per vial)Stability:Stable in culture over a minimum of 10 passagesApplication: Drug screening and biological assaysFreeze medium:70% RMPI-1640 + 20% FBS + 10% DMSOPropagation medium: RPMI 1640 + 10% FBSSelection marker: PuromycinMorphology: Mostly single, round (some polymorph) cells in suspensionSubculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/mlIncubation: 37 °C with 5% CO2Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSODoubling time: Approximately 20 hoursMycoplasma status:NegativeBiosafety level: 1 Storage:Liquid nitrogen immediately upon receivingII．BackgroundEML4-ALK, echinoderm microtubule associated protein like 4 - anaplastic lymphoma kinase, is an abnormal protein with transforming activity found in some primary lung malignant tumor due to the fusion of abnormalconfiguration of DNA wherein the echinoderm microtubule-associated protein-like 4 (EML4) gene is fused to theanaplastic lymphoma kinase (ALK) gene; EML4-ALK and its mutants can promote and maintain the malignantbehavior of the cancer cells. The identification of ALK as a driver gene has led to the rapid development ofanticancer therapeutics agents, including crizotinib, ceritinib and Brigatinib.Ba/F3 cell, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases andtheir inhibitors, because some protein kinases can render the Ba/F3 cells to be depended on the activation of thekinases instead of IL-3 supplement, while their inhibitors can antagonize the kinase-dependent growth effects.III．Cell Line GenerationBa/F3 EML4-ALK-G1202R cell Line was generated using retrovirus vector expressing human EML4-ALK G1202Rsequence

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