BaF3_BCR-ABL-T315I_Cell_Line激酶靶點穩轉細胞株-BioVector NTCC典型培養物保藏中心

Cell line name:Ba/F3 BCR-ABL-T315I Cell LineGene ID/Accession #: Host cell lineMouse Ba/F3 cell line Cell type: Pro-B cellsDescription: Stable Ba/F3 clone expressing exogenous BCR-ABL gene bearing T315I point mutationin ABL part.Quantity:One vial of frozen cells (5X106 per vial)Stability:Stable in culture over a minimum of 10 passagesApplication: Drug screening and biological assaysFreeze medium:70% RMPI-1640 + 20% FBS + 10% DMSOPropagation medium: RPMI 1640 + 10% FBSSelection marker: PuromycinMorphology: Mostly single, round (some polymorph) cells in suspensionSubculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/mlIncubation: 37 °C with 5% CO2Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSODoubling time: Approximately 20 hoursMycoplasma status:NegativeBiosafety level: 1 Storage:Liquid nitrogen immediately upon receivingII．BackgroundBCR-ABL, breakpoint cluster region–Abelson murine leukemia viral oncogene homolog 1, is an abnormal protein mostly found in chronic myelogenous leukemia (CML) due to the fusion of abnormal configuration of DNA wherethe abl gene is fused to the BCR gene (The Philadelphia chromosome); BCR-ABL and its mutants can promote andmaintain the malignant behavior of the cancer cells. The identification of BCR-ABL as a driver gene has led to therapid development of anticancer therapeutics agents, including Imatinib, Dasatinib, Ponatinib and Nilotinib。Ba/F3 cell, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases andtheir inhibitors, because some protein kinases can render the Ba/F3 cells to be depended on the activation of thekinases instead of IL-3 supplement, while their inhibitors can antagonize the kinase-dependent growth effects.III．Cell Line GenerationBa/F3 BCR-ABL cell Line was generated using retrovirus vector expressing human BCR-ABL fusion sequence

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