pEXG2-ΔpcrV2 plasmid vector質粒載體

RP2318 PAO1F ΔexsE ΔexoT ΔexoY [1]
RP2349 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) [1]
RP2888 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) ΔpopD This study
RP2996 PAO1F ΔexsE ΔexoT ΔexoY Δpcr1 This study
RP3624 PAO1F ΔexsE ΔexoT ΔexoY ΔpcrHpopBD This study
RP3714 PAO1F ΔexsE ΔexoT ΔexoY ΔpcrHpopBD lcrV(N)-pcrV(C) V1 This study
RP6592 PAO1F ΔexsE ΔexoT ΔexoY ΔpcrHpopBD lpv72 V2 This study
RP5891 PAO1F ΔexsE ΔexoT ΔexoY ΔpcrHpopBD pcrV-α6-lcrV V3 This study
RP6225 PAO1F ΔexsE ΔexoT ΔexoY ΔpcrHpopBD pcrV-α456-lcrV V4 This study
RP3670 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-)
ΔpcrHpopBD
This study
RP6369 PAO1F ?exsE ?exoT ?exoY ?pcr1 “S+” ?pcrHpopBD This study
RP6370 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-)
ΔpcrHpopBD Δpcr1
This study
RP6641 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-)
pcrV(Q87C) ΔpopD
This study
RP8134 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-)
popB(A280C)
This study
RP8136 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-)
popD(R243C)
This study
RP8766 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) Δpcr1
popD(R243C)
This study
RP9266 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) Δpcr1 This study
RP9268 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) ΔpcrV2 This study
RP9270 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) PYD268 This study
RP9272 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) lcrV(N)-
pcrV(C)
This study
RP9274 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) lcrV(N)-
pcrV(C) PYD268
This study
RP9276 PAO1F ΔexsE ΔexoT ΔexoY exoS(GAP-/ADPR-) Δpcr1
PYD268
D4 This study
Plasmids
Plasmid Relevant features Reference
pPSV37 colE1 origin, gentR, PA origin, oriT, lacUV5 promoter, lacIq,
stops in every reading frame preceding the MCS and T7
terminator following the MCS (relative to the lacUV5
promoter)
[2]
pEXG2 allelic exchange vector, colE1 origin, oriT, gentamicin
resistance, sacB
[3]
pEXG2-ΔpcrV2 delete codons 4-180 of ?pcrV2 [1]
pEXG2-Δpcr1 delete codons 10–81 of pcr1 [4]
pEXG2-pcrV(Q87C) introduce Q87C mutation into pcrV This study
pEXG2-popB(A280C) introduce A280C mutation into popB This study
pEXG2-popD(R243C) introduce R243C mutation into popD This study
pEXG2-lcrV(N)-pcrV(C) replace pcrV with lcrV(1-145)-pcrV(124-294) hybrid V1 This study
pEXG2-LPV72 replace pcrV with lcrV(1-G87)-pcrV(G72-294) hybrid V2 This study
pEXG2-pcrV-α6-lcrV replace pcrV with pcrV(1-110)-lcrV(133-144)-pcrV(123-294)
fusion replacing α6
V3 This study
pEXG2-pcrV-α456-lcrV pcrV with codons A84-E96 replaced by lcrV codons L105-
A117, and pcrV V116-Q124 replaced by lcrV V138-H146
(portions of α4, α5, and α6)
V4
pEXG2-PYD268 replace popD with popD(1-268)-yopD(280-306) hybrid D4 This study
pP37-lcrHpcrH translationally coupled lcrH and pcrH genes This study
pP37-lcrHpcrH-popBD plasmid encoding wild type popB and popD under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-popByopD plasmid encoding wild type popB and yopD under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBpopD plasmid encoding wild type yopB and popD under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBD plasmid encoding wild type yopB and yopD under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-popBYPD110 plasmid encoding popB and a yopD(1-118)-popD(110-295)
fusion under control of the lacUV5 promoter in pPSV37
D1 This study
2
pP37-popD Plasmid encoding wild type PopD under control of the lacUV5
promoter in pPSV37
[4]
pP37-popD(A292C) plasmid encoding popD with codon Ala292 changed to Cys
under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-popBYPD246 plasmid encoding popB and a yopD(1-256)-popD(246-295)
fusion under control of the lacUV5 promoter in pPSV37
D2 This study
pP37-lcrHpcrH-popBYPD269 plasmid encoding popB and a yopD(1-279)-popD(269-295)
fusion under control of the lacUV5 promoter in pPSV37
D3 This study
pP37-lcrHpcrHpopByopD(F303A)
plasmid encoding popB and yopD(F330A) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(R268Q)
plasmid encoding popB and yopD(R268Q) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(E289Q)
plasmid encoding popB and yopD(E289Q) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(V292T)
plasmid encoding popB and yopD(V292T) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(S293Q)
plasmid encoding popB and yopD(S293Q) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(T296N)
plasmid encoding popB and yopD(T296N) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(H297Q)
plasmid encoding popB and yopD(H297Q) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(M299W)
plasmid encoding popB and yopD(M299W) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopByopD(K300R)
plasmid encoding popB and yopD(K300R) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopB(A280C)popD
plasmid encoding popB(A280C) and popD under control of
the lacUV5 promoter in pPSV37
This study
pP37-popD172-4V plasmid encoding popD into which four copies of the VSV-G
epitope tag were inserted after codon 172 under control of
the lacUV5 promoter in pPSV37
This study
pP37-popD(R243C)172-4V plasmid encoding popD(R243C) into which four copies of the
VSV-G epitope tag were inserted after codon 172 under
control of the lacUV5 promoter in pPSV37
This study
pP37-popB225-4V plasmid encoding popB into which four copies of the VSV-G
epitope tag were inserted after codon 225 under control of
the lacUV5 promoter in pPSV37
This study
pP37-popB(A280C)225-4V plasmid encoding popB(A280C) into which four copies of the
VSV-G epitope tag were inserted after codon 225 under
control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrHpopBpopD(R243C)
plasmid encoding popB and popD(R243C) under control of
the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBYPD110 plasmid encoding yopB and a yopD(1-118)-popD(110-295)
fusion under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBYPD246 plasmid encoding yopB and a yopD(1-256)-popD(246-295)
fusion under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBYPD269 plasmid encoding yopB and a yopD(1-279)-popD(269-295)
fusion under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBPYD109 plasmid encoding yopB and a popD(1-109)-yopD(119-306)
fusion under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBPYD228 plasmid encoding yopB and a popD(1-228)-yopD(240-306)
fusion under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-yopBPYD245 plasmid encoding yopB and a popD(1-245)-yopD(257-306)
fusion under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-YPB108popD plasmid encoding a yopB(1-103)-popB(108-390) hybrid and
popD under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-YPB165popD plasmid encoding a yopB(1-160)-popB(165-390) hybrid and
popD under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-YPB255popD plasmid encoding a yopB(1-251)-popB(255-390) hybrid and
popD under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-YPB274popD plasmid encoding a yopB(1-269)-popB(274-390) hybrid and
popD under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-PYB206popD plasmid encoding a popB(1-206)-yopB(203-390) hybrid and
popD under control of the lacUV5 promoter in pPSV37
This study
pP37-lcrHpcrH-PYB297popD plasmid encoding a popB(1-297)-yopB(309-390) hybrid and
popD under control of the lacUV5 promoter in pPSV37
This study

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