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pDB2688
Plasmid Linker Gene inserted in at SaBI/Not- Stability afterasmi nsertion site in US-region site in UL-region 30 generationUS-regionLEU2 100pDB2688 XcmI after REP2 LEU2 100pDB2812 HgalafterFLP LEU2 100pDB2817 XznI in REP2 LEU2 1pDB2690 XcmI after REP2 PDI1 (SKQ2n) LEU2 33pDB2711 Xcml after REP2 PDI1 (SKQ2n) LEU2 rTf 2
pDB2814 Bell FLP (1-353) 67% 64%pDB2823 XC717I FLP (1-382) 64% 53%pDB2812 pDB2813 HgaI Inverted Repeat 100% 100%pDB28OS
Plasmid(s) ]fInsertion Additional Relative StabilitySite Details Set I Set 2-Control 99% 100%pDB2 817 pDB28918 A'innI REP2 (1-244) 39% 16%pDB2787 ApaIJT4 pol. REP2 (1-269) 45% 0%pDB2788 ApaI REP2 (1-271) 33% 0%pDB2688 XcrnI Inverted Repeat 100% 100%pDB280 pDB2906 FspI Inverted Repeat 100% 10 0%
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