pLOW plasmid金黃色葡萄球菌表達質粒載體

Plasmid pLOW, designed for regulated expression of S. aureus proteins. The MCS is shown in full, with the reading frame indicated by the triplet arrangement of the nucleotide sequence. Only unique restriction sites are shown. Genes: ApR, E. coli blaM gene for b-lactamase; ColEI-HC, high-copy-number E. coli plasmid origin of replication; EmR, S. aureus gene originating from plasmid pE194 and encoding Em-resistance gene ermC; pSK41 ori, lowcopy-number S. aureus origin of replication; LacI, lac repressor protein constitutively expressed from the penicillinase promoter Ppcn. (b) Plasmid pLOW-GFP, designed for producing protein– GFP fusions in S. aureus. GFP, gfpmut-1 gene encoding GFP.  

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