pMir-Target miRNA熒光報告質粒載體

pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3’UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity.High sensitivity of Luciferase assay due to IRES-driven luciferase cassetteRFP as a reporter for transfection monitoring and normalizationNeomycin selection marker for stable cell establishmentCompatible with Precision shuttling systemCan serve as a control for target validation experiments.3-UTR Clones for MicroRNA target validation

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