IDG-SW3 Bone Cell Line

Cell line IDG-SW3 replicates osteoblast to late osteocyte differentiation in vitro and increases bone formation in vivo. Highlights: Express series markers from early to late osteocytes, including: Dmp1-GFP, E11/gp38, SOST/sclerostin, and FGF-23Maintained in non-differentiated state at 33°C with IFN-g present - allows for large scale production without the loss of phenotypeSame gene expression as primary cells when cultured at 37°C with no IFN-g presentClonal cells - homogeneous, and at the same stage of differentiationCan be maintained at both 2D and 3D culturesLong term viability (35-50 days) Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line should be useful study osteoblast to osteocyte transition, mechanisms for biomineralization osteocyte function and regulation of SOST/sclerostin and FGF-23.
Product Type: Cell Line Name: IDG-SW3 Cell Type: Osteoblast/early osteocyte/late osteocyte Accession ID: CVCL_0P23 Organism: Mouse Source: Long bone Morphology: Adherent osteoblast-like cell Biosafety Level: I Subculturing: See: IDG-SW3 Cell Line Protocol Growth Conditions: Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; 1% penicillin-streptomycin; Recombinant Mouse Interferon-gamma (INF-γ) 50 U/ml. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Incubate at 33°C with 5% CO2 Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; 1% penicillin-streptomycin; 50μg/ml Ascorbic Acid and 4mM β-glycerophosphate. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Incubate at 37°C with 8% CO2. Cryopreservation: 60% AlphaMEM, 30% FBS, 10% DMSO, at 1-2 x 10^6 cells/vial/1ml

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