L6 Rat Myoblast Cell Line

Immortalized rat skeletal (L6) myoblast cell line that was selected for high fusion potential and endogenous expression of GLUT4 in the myotube stage. Highlights: Differentiate with high reliability into a myotube muscle cell phenotype that naturally expresses the GLUT4 glucose transporter proteinSignificant insulin-stimulated glucose uptake biological responseUseful for novel anti-diabetic compound screeningAmendable for transient transfection by plasmid-based gene transfer and viral infection protocols (retrovirus and adenovirus) Insulin stimulation of glucose transport in skeletal muscle results mainly due to translocation of the glucose transporter GLUT4 to the cell surface. L6 cells, originally derived from rat skeletal muscle, propagate as mononucleated myoblasts but can differentiate into multinucleated primary myotubes. The myotubes express several proteins typical of skeletal muscle including the GLUT4 glucose transporter. Insulin stimulates glucose uptake with high sensitivity and maximal responsiveness only in differentiated L6 myotubes and GLUT4 expression parallels the acquisition of these characteristics as the L6 cells differentiate. These features of L6 myotubes are important since GLUT4 is responsible for insulin-dependent glucose uptake in mature skeletal muscle.
Product Type: Cell Line Name: L6 Cell Type: Skeletal muscle Organism: Rat Accession ID: CVCL_0385 Source: Quadriceps Morphology: Myoblast/Myotube Biosafety Level: II Subculturing: 2-3 days Growth Conditions: MEM-α (with Ribonucleosides and Deoxyribonucleosides) +10% FBS (See protocol below) Cryopreservation: MEM-α +10% FBS+ 10% DMSO

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