LOBSTR E. coli Expression Strain

LOBSTR (low background strain) is an E. coli strain for the expression of recombinant polyhistidine-tagged proteins. This strain has been optimized for one-step downstream polyhistidine-tag affinity purification and is ideal for poorly expressing proteins. Highlights: Yields recombinant polyhistidine-tagged protein of higher purity by reducing the contamination by E. coli ArnA and SlyDAllows for a one-step purification to eliminate the major E. coli contaminantsBased on BL21(DE3) - use the same as other commercially available competent cellsIdeal for purification of challenging low-expressing protein targetsAlso available: BL21(DE3)-RIL version - Contains extra copies of the argU, ileY, and leuW tRNA genes, as well as a chloramphenicol marker A major drawback of polyhistidine-tag affinity purification of proteins expressed in E. coli is the presence of naturally histidine-rich proteins, resulting in co-purification of these contaminants. In LOBSTR, ArnA and SlyD, the two most common E. coli contaminants have been modified based on surface engineering. LOBSTR maintains normal cell growth but significantly reduces the polyhistidine-tag binding affinities of ArnA and SlyD. Compared to other expression strains, LOBSTR yields recombinant protein of higher purity, allowing for one-step purifications of low expressing recombinant proteins.
Product Type: Bacteria Name: LOBSTR E. coli Expression Strain Cell Type: Chemically competent (CaCl2method) Organism: E. coli BL21(DE3) Competency: >1x106cfu/ug DNA Growth Conditions: Standard E. coli Growth Media (LB, SOC, etc.) at 37C Transformation: Standard heatshock protocol (42C for 20 seconds) Induction: IPTG up to 1mM Comments: Derived from E. coli BL21(DE3)

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