Collagen I (Rat tail derived)

rMC-1 immortalized cells constitutively express GFAP (glial fibrillary acidic protein) and CRALBP (cellular retinaldehyde-binding protein) which are present in Müller cells. Highlights: Constitutively express GFAP and CRALBPImmortalized by transfecting primary Müller cell cultures from adult rat retinas with a viral oncogeneExpress both induced and basal markers found in primary Müller cell culturesHighly useful for preclinical studies in diabetic retinopathyCell line validation performed - See: Pfeffer BA, et al. Exp Eye Res. 2016 Feb 12;145:297-316. Müller cells are the most abundant type of glial cells found in the vertebrate retina, and perform diverse functions that support the activity of retinal neurons. This immortalized cell line facilitates gene expression studies in Müller cells and improves the understanding of Müller cell-neuron interactions.
Product Type: Cell Line Name: rMC-1 Cell Type: Retinal Müller (glial) cell Accession ID: CVCL_8140 Organism: Rat Source: Retina Morphology: Flat Biosafety Level: BSL1 Subculturing: Harvest cells with 0.05% trypsin for 5 min and replate Growth Conditions: DMEM (high glucose, (+) L-Glutamine, (-) Sodium pyruvate) with 10% FBS and 1X pen/strep, with additional L-glutamine (2mM). Cryopreservation: DMEM with 10% DMSO and 10%FBS

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