Hepatocyte Culture Media

Immortalized cell line HEK293 that stably expresses the type 1 ryanodine receptor (RyR1). Highlights: Cell line that stably expresses RyR1RyR1 expression validated by immunoblotting and immunocytochemistry techniquesRyR1 function validated by calcium release in response to addition of RyR1 agonist 4-cholo-m-cresolRyR1 single channel activity validated in nuclear patch clamp experiments The type I ryanodine receptor (RyR1), also known as skeletal muscle calcium release channel or skeletal muscle-type ryanodine receptor, is a protein found primarily in skeletal muscle. The main role of this protein is to form an ion channel that transports calcium ions from an intracellular sarco/endoplasmic reticulum storage compartment to the cytoplasm. Additionally, the RyR1 protein plays a critical role in muscles that are used for movement by causing them to contract.
Product Type: Cell Line Name: HEK293-RyR1 Cell Type: HEK293 Accession ID: CVCL_0P75 Organism: Human Morphology: Flat/Circular Source: Kidney Biosafety Level: BSL2 Subculturing: Usually a 1:5 or 1:10 dilution will be 80-90% confluent in 3-4 days Growth Conditions: DMEM (High Glucose), 10% FBS, 2mM L-Glutamine, 1% Pen/Strep, 100μg/ml Hygromycin Cryopreservation: 90% complete media with 10% DMSO

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