IDG-SW3 Bone Cell Line

Stably transfected Sf9 cells which emit eGFP upon infection with baculovirus. Highlights Stably transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoterUsed in titration assay to determine baculovirus titer in lieu of CPE or Plaque assays96-well plate format end-point dilution assay allows for titers to be determined in three days using a fluorescence microscope The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
Product Type: Cell Line Name: Sf-9 ET Alternative Name(s): SF-9ET; Sf-9 Easy Titer; Sf9-ET; Sf9-Easy Titer; Sf9 Easy Titer Cell Type: Epithelial-like Organism: Spodoptera frugiperda Accession ID: CVCL_1D71 Source: Ovary Morphology: Rounded Sphere in suspension and when adhered Biosafety Level: 2 Growth Conditions: Sf-900 III SFM + 50 micrograms/ml Geneticin Subculturing: Seed at 1 X 106 cells/ml for 3-day transfer, 8 X 105 cells/ml for 4-day transfer Cryopreservation: 80% HyClone SFX, 10% FBS, 10% DMSO Donor Attributes: Female Comments: Transfected with: UniProtKB; P42212; GFP.

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