L6-GLUT4myc Rat Myoblast Cell Line

Trichoplusia ni cell line derived from BTI-Tn-5B1-4, adapted for suspension growth and serum-free growth. Highlights Used for protein expression by baculovirus infectionCan grow efficiently at 21 or 27 C in adherent or suspension conditions The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
Product Type: Cell Line Name: Tni-FNL Cell Type: Insect Organism: Trichoplusia ni Accession ID: CVCL_RY32 Source: Ovary Morphology: Rounded spheres Biosafety Level: BSL2 Growth Conditions: Sf-900 III Serum Free Media; 21 hour doubling time, Max Growth 5 x 106 cells/ml Subculturing: 5 x 105 cells/ml for 4 day growth, 6 x 105 cells/ml for 3 day growth Cryopreservation: 80% Sf-900 III; 10% FBS; 10% DMSO Comments: Use of 10 units/ml of Heparin can be used on highly aggregated cultures to disperse aggregation

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