LOBSTR E. coli Expression Strain

Permanent cell line U-251MG derived from a human malignant glioblastoma multiforme. Highlights: Continue to express glial fibrillary acidic protein (GFAP), over many cell passagesExpresses epidermal growth factor receptor (EGFR) receptors, for inhibition studiesIsolated from 75 year old male with astrocytoma, grade III-IV malignant tumor by explant technique Astrocytomas are a type of cancer in the brain that originate in a particular kind of glial cells, called astrocytes. High-grade astrocytomas, also called glioblastoma multiforme are the most malignant of all brain tumors.
Product Type: Cell Line Name: U-251 MG Cell Type: Glioblastoma Accession ID: CVCL_0021 Organism: Human Source: Brain Morphology: Fibroblastic pattern Biosafety Level: 2 Growth Conditions: Improved MEM Zinc Option +10% FBS, Penn/strep, amphotericin B Subculturing: Split 1:2 or 1:3 every 3 days using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37C. Cryopreservation: 90% (MEM Zinc Option with 20% FBS) + 10% DMSO

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