U-698-M cell line細胞株

Cell line:U-698-MNTCC no.:ACC 4Species:human (Homo sapiens)Cell type:B cell lymphomaOrigin:established from the involved tonsil of a 7-year-old boy with lymphoblastic lymphoma at diagnosis prior to therapy in 1972; cells were described in the literature to be EBV-negativeReference(s):14806, 14807, 15230Biosafety level:1Permissions and restrictions:A NTCC Cell Culture Data:Morphology:single or clumped cells in suspension; some cells adhere to plastic; image ; imageMedium:90% RPMI 1640 + 10% h.i. FBSSubculture:seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; some cells adhere to plastic, but can be detached mechanically (trypsin not necessary)Incubation:at 37 °C with 5% CO2Doubling time:ca. 48 hoursHarvest:maximal density at 1.5 x 106 cells/mlStorage:frozen with 70% medium, 20% FBS, 10% DMSO NTCC Scientific Data:Mycoplasma:negative in DAPI, microbiological culture, PCR assaysImmunology:CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; imageFingerprint:multiplex PCR of minisatellite markers revealed a unique DNA profileSpecies:confirmed originally as human with IEF of AST, NP and later by STR fingerprintingCytogenetics:human hyperdiploid karyotype with 5.5% polyploidy - 49(44-50)<2n>XY, +3, +7, -14, +mar, dup(1)(q43q21.2), der(2)t(2;3)(p16;p11), add(3)(p11), del(6)(q15q22), del(9)(p22), dup(11)(q23q13), add(13)(p12), add(16)(q24) - carries large submetacentric dup(1) markerViruses:ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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