AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid腺相關病毒質粒及AAV病毒

Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore. The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled.

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