pShuttle-IRES-hrGFP-1腺病毒表達載體質粒圖譜序列抗性價格報價Biovector NTCC Inc.
- 價 格：￥1920
- 貨 號：BiovectorShuttle-IRES-hrGFP-1
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
載體基本信息
| 出品公司: | Agilent |
|---|---|
| 載體名稱: | pShuttle-IRES-hrGFP-1 |
| 質粒類型: | 腺病毒表達載體，哺乳動物細胞表達載體 |
| 高拷貝/低拷貝: | 低拷貝 |
| 啟動子: | CMV |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 8.9 kb |
| 5' 測序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 測序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 載體標簽: | -- |
| 載體抗性: | 卡那 |
| 備注: | 載體內包含有IRES序列和hrGFP序列。 |
| 產品目錄號: | 240081 |
| 穩定性: | / |
| 組成型: | -- |
| 病毒/非病毒: | 腺病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG? epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
載體序列
載體質粒圖譜pdf版和相關資料下載
載體應用舉例
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