COLO 853 cell line細胞株ICLC HTL96001

Cell line name COLO 853
Accession number ICLC HTL96001
Brief description Species: human; Tissue: lymph node; Tumor: melanoma
Description Species: human male 45 years old; Tissue: lymph node; Tumor: melanoma
Depositor Obtained from ECACC
Reference paper Unknown
Morphology and growth continuous culture, grown as monolayer, morphology epithelial
Culture conditions RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5x104 cells/cm2; 37C, 5% CO2
Properties melanin production
Distribution Cell line available for distribution. For non-commercial investigative use only
Hazard
Species validation Validated by isoenzymes: confirmed as human with AST, MD
Freezing medium Culture medium + 50% FBS + 10% DMSO
Validation assays Identification confirmed by Short Tandem Repeat (STR) profile.
Further bibliography Cancer Genet Cytogenet 1993;69:108-112 - PMID: 8402545

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