BSL 1

Morphology

After 3 days at 25°C colonies cream-coloured, smooth, dull, with a mycelial border forming after prolonged incubation. Yeast cells globose to short-ovoid to long-ovoid 3·0-5·5 x 4·0-9·0 μm, single or budding, sometimes forming short chains with mycelium often present in older cultures.

Comments

Genome sequencing strain (Broad Institute, USA).

mitochondrial genome sequenced strain

Medium

Medium 28: Emmons' modification of Sabouraud's agar

Medium 200: YM agar or YM broth

Medium 1245: YEPD

Temperature

30°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.

1. To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.

2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 μL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.

3. Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

Deposited as

Candida tropicalis

Depositors

C Pujol

Chain of custody

F Odds , C Pujol University of Iowa

Special collection

NSF - Mycology

Cross references

GenBank AAFN00000000 Candida tropicalis MYA-3404, whole genome shotgun sequencing project