Cell line:

SKM-1

NTCCno.:

NTCC-D547

Species:

human (Homo sapiens)

Cell type:

acute myeloid leukemia

Origin:

established from the peripheral blood of a 76-year-old Japanese man with acute monoblastic leukemia (AML M5) in 1989 following myelodysplastic syndromes (MDS)

Biosafety level:

1

NTCCCell Culture Data:

Morphology:

round cells growing singly in suspension;

Image:

Medium:

80% RPMI 1640 + 15-20% h.i. FBS

Subculture:

seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; maintain at ca. 0.4-2.0 x 106 cells/ml

Incubation:

at 37 °C with 5% CO2

Doubling time:

ca. 48 hours

Harvest:

cell harvest of ca. 2.5 x 106 cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

NTCCScientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD4 (+), CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image

Fingerprint:

multiplex PCR of minisatellite markers revealed a unique DNA profile

Species:

confirmed as human by cytogenetics

Cytogenetics:

human hypodiploid karyotype with 8% polyploidy - 43(38-43)<2n>XY, +1, -12, -14, -20, -21, t(1;19)(q21;q13), del(2)(p11), del(9)(q12), add(17)(p1?) - sideline with idem, der(10)t(10;?21)(p11;q11), -21 - consistent with published karyotype

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -