CET 1019 HD-hygro-SceI Plasmid UCOE表達系統載體質粒

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HD-hygro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AD-hygro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HD-hygro-SceI and CET 1019 AD-hygro-SceI vectors possess the following features:

? A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
? A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3’ end formation
? A hygromycin antibiotic resistance gene for selection of stably transfected mammalian cells
? A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
? An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes

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