pC3C Phage Display Plasmids噬菌體展示質粒載體Fab系統

Phagemid pC3C for phage display of Fab with human constant domains and accompanying plasmid pC3C-His for the E. coli expression of Fab with a C-terminal hexa-histidine tag.

Highlights:

Yield 100 ug to 2 mg of protein for downstream evaluation and characterization of Fab binding propertiesOnly 2 PCR steps required for library assembly (compared to 3 PCR steps for pComb3H and pComb3X phage display vectors)pC3C-His version contains hexa-tag for easy Fab purification
The Fab molecule was the first generated antibody fragment and still dominates basic research and clinical applications. New phage display vectors were designed to generate and select Fab libraries with human constant domains. These vectors facilitate bacterial expression of human humanized and chimeric nonhuman/human Fab antibody fragments. They differ from currently available pComb3H and pComb3X phage display vectors by assembling human and chimeric nonhuman/human Fab libraries in two rather than three PCR steps. As a result these novel constructs retain the initial variable light and heavy chain sequences and improve the resulting Fab library's complexity in terms of number diversity and affinity. These constructs were developed with and without a His tag and yield approximately 100 ug to 2 mg of protein which can be used for evaluation and characterization of Fab binding properties such as affinity and specificity. Notably the His tag provides a handle to easily purify Fab.
Product Type: Plasmid
Gene/insert name: VL-CL-VH cassette of a Fab that binds human GPA33
Organism: chimeric rabbit/human
Antibiotic Resistance: Ampicillin, carbenicillin
Fusion Tag(s): hemagglutinin decapeptide (YPYDVPDYAS), pC3C-His also contains C-termnial hexa-his tag
Buffer: Water
Grow in E. coli at 37 C: Yes
Cloning Site 5': SfiI GGCCcaggcGGCC
Cloning Site 3': SfiI GGCCccgtcGGCC
Insert Size: ca. 1,150 bp
Vector Backbone and Size: 4,706 bp

Supplier來源:BioVector NTCC Inc.
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