ES-E14TG2a cell line小鼠胚胎干細胞

Keywords: Mouse (strain 129/Ola) embryonic stem cellCell Line Description: Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.Species: MouseTissue of Origin: Embryo blastocystCellType: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells ECACC Catalogue 86032003 (irradiated or treated with mitomycin C to prevent cell division. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.Growth Mode: AdherentBiosafety Information: 1Culture Medium: DMEM + 2mM Glutamine + 1% NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Foetal Bovine Serum. Renewal of medium every 2-3 days is required.Depositor: Professor T Doetschman, Institute for Collaborative Research, University of Arizona, Tucson, Arizona 85721-0036. Originator: Professor M Hooper, University of Edinburgh EH4 2XU, UK.Originator: NoCountry: USAReferences: Smith & Hooper (1987) Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9. PMID: 3569655.

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