pASK-IBA37plus plasmid vector大腸桿菌四環(huán)素誘導(dǎo)表達(dá)質(zhì)粒載體

Description Expression plasmid. The expression cassette is under transcriptional control of
the tetracycline promoter/operator. The expressed recombinant protein will be
localized in the cytoplasm. Affinity tag 6xHistidine-tag for the purification of recombinant protein. The affinity tag is
fused to the N-terminus of the recombinant protein and can be removed by
cleavage with factor Xa. Bacterial Expression Expression is induced upon addition of 200 μg anhydrotetracycline (order no.:
2-0401-001; 2-0401-002) per 1 liter E. coli shaking culture (A550 = 0.5). Expression strain Any E. coli strain. The tet-promoter works independently from the genetic
background of E. coli. Resistance Ampicillin


Supplier來源:BioVector NTCC Inc.
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