pcDNA3,1-GFP287-FokI and pcDNA3,1-GFP296-FokI plasmid vector質粒載體

the results where T18 cells were transfected with pcDNA3,1-GFP287-FokI and pcDNA3,1-GFP296-FokI plasmids encoding ZFP nucleases and plasmid pCR (R) 4-TOPO-GFP donor5, eGFP expression was induced with doxycycline and cells were arrested in G2 with nocodazole (Figure 30) or vinblastine (Figure 31). Both figures show FACS traces, in which cells exhibiting eGFP fluorescence are represented in the lower right part of the trace (identified as Region E, which is the part of Table 4 below the curve). For the transfected cells that had been treated with nocodazole, 5.35% of the cells exhibited GFP fluorescence, indicative of correction of the mutant chromosomal eGFP gene (Figure 30), while 6.7% of the cells treated with vinblastine were subjected to correction of the eGFP gene (Figure 31). These results are summarized, along with additional control experiments

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