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首頁 ? pBAM1(34) plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心

pBAM1(34) plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心

  • 價  格:¥49865
  • 貨  號:pBAM1(34)
  • 產  地:北京
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BioVector NTCC典型培養物保藏中心
聯系人:Dr.Xu, Biovector NTCC Inc.

電話:400-800-2947 工作QQ:1843439339 (微信同號)

郵件:Biovector@163.com

手機:18901268599

地址:北京

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pBAM1(34) plasmid vector質粒載體

The functional DNA sequences present in the natural bacterial transposon Tn5 have been methodically edited and refactored for the production of a multi-purpose genetic tool named pBAM1, which allows a range of manipulations in the genome of Gram-negative bacteria. This all-synthetic construct enhances the power of mini-transposon vectors for either de-construction or re-construction of phenotypes á la carte by incorporating features inspired in systems engineering: modularity, re-usability, minimization, and compatibility with other genetic tools. pBAM1 bears an streamlined, restriction site-freed and narrow-host range replication frame bearing the sequences of R6K oriV, oriT and an ampicillin resistance marker. These go along with a business module that contains a host-independent and hyperactive transposition platform for in vivo or in vitro insertion of desired DNA into the genome of the target bacterium. All functional sequences were standardized for a straightforward replacement by equivalent counterparts, if required. pBAM1 can be delivered into recipient cells by either mating or electroporation, producing transposon insertion frequencies of 1.8 × 10-3 and 1.02 × 10-7, respectively in the soil bacterium Pseudomonas putida. Analyses of the resulting clones revealed a 100% of unique transposition events and virtually no-cointegration of the donor plasmid within the target genome.

Supplier來源:BioVector NTCC Inc.
TEL電話:+86-010-53513060
Website網址: http://www.biovector.net

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