Clearcoli k12 E.coli competent cells無內毒素大腸桿菌菌株-感受態細胞

ClearColi? K-12 Electrocompetent Cells
Eliminate endotoxins at the source
? Genetically modifi ed LPS does not trigger endotoxic response in
mammalian cells
? Plasmid yields similar to DH10B cells
? Ideal for mammalian transfection and protein expression
? No expensive endotoxin-free plasmid preps necessary
Instead of removing lipopolysaccharide (LPS) contamination from your
plasmid DNA preparations, eliminate the LPS at the source. ClearColi?
K-12 cells are the fi rst commercially available competent cells with a
modifi ed LPS that produces functionally clean plasmids. ClearColi cells
lack outer membrane agonists for hTLR4/MD-2 activation; therefore,
activation of hTLR4/MD-2 signalling by ClearColi? is several orders of
magnitude lower compared with E. coli wild-type cells, and plasmids
prepared from ClearColi are virtually free of endotoxic activity.
Standard, non-endotoxin free plasmid prep methods can be used to
produce clean plasmids ready for use in most downstream applications
without eliciting an endotoxic response
Applications of ClearColi K-12 cells:
? Cloning and plasmid production
? Mammalian transfection without endotoxin cleanup
? Transient mammalian protein expression
GENOTYPE:
F– λ- ?endA ?recA frr181 msbA52 ?gutQ ?kdsD ?lpxL ?lpxM ?pagP ?lpxP ?eptA

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