CHO-K1/M4/Ga15 Stable Cell Line M受體穩(wěn)轉(zhuǎn)細(xì)胞株

Description
Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M4 muscarinic receptor couples to Gi/o to inhibit cAMP production. GenScript co-transfected human M4 with Gα15 in the CHO-K1 which supports high levels of recombinant M4 expression on the cell surface and contains high levels of Gα15 to couple the receptor to the calcium signaling pathway.

Synonyms
M4 receptor, m4, HM3, acetylcholine receptor, muscarinic 4, cholinergic receptor, muscarin 4, muscarinic acetylcholine receptor M4, Chrm-4, muscarinic acetylcholine receptor 4
Species Human
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Properties
Storage Liquid nitrogen immediately upon delivery
Applications
Functional assays for M4 receptor

Supplier來源:BioVector NTCC Inc.
TEL電話:+86-010-53513060
Website網(wǎng)址: http://www.biovector.net