Description: JU77 is a human mesothelioma cell line derived from a male patient. The patient had known exposure to crocidolite asbestos. The culture was developed from a pleural effusion sample. Malignant mesothelioma was diagnosed through analysis of pleural effusion fluid.




Organism: Human (Homo sapiens)




Strain: N/A




Tissue: Pleural fluid




Growth Properties: Adherent




Morphology: Epithelial-like; cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid “cobblestone-like mat”.




Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 5% Foetal Calf Serum




Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.




Subculturing Procedure:



Medium Renewal: 2-3 times per week.




Subcultivation Ratio: 1:8, Seeding density 0.8 x104cells/cm2. Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.




Culture Conditions: Incubate the culture at 37°C with 5% CO2.




Cryoprotectant Medium: 10% DMSO + 90% FCS




Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.




Additional Information: JU77 cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Not tumourgenic in nude mice.



Medium:
Growth Condition:

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