Description: MM127 is an adherent melanoma cell line; it was established in 1970 from a male patient. It was derived from a subcutaneous metastatic melanoma.



Description Key Words: Human melanoma male



Also Known As: No other names



Organism: Human (Homo sapiens)



Strain: N/A



Tissue: Skin



Growth Properties: Adherent



Morphology: Epithelial



Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 10% Foetal Calf Serum



Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.



Subculturing Procedure:



Medium Renewal: 2-3 times per week.



Subcultivation Ratio: 1:8, Seeding density 0.6 x104cells/cm2. Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.



Culture Conditions: Incubate the culture at 37°C with 5% CO2.



Cryoprotectant Medium: 10% DMSO + 90% FCS



Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.



Additional Information:



Mutations:

- Homozygous deletion CDKN2A

- G13R mutation NRas



Medium:
Growth Condition:

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