Description: Melanoma cell line from lymph node



Description Key Words: Human melanoma



Also Known As: No other names



Organism: Human (Homo sapiens)



Tissue: Skin



Growth Properties: Adherent



Morphology: Epithelial



Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 10% Foetal Calf Serum (FCS)



Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.



Subculturing Procedure:



Medium Renewal:



Subcultivation Ratio: 1:4, Seeding density 0.8 x104 cells/cm2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard, the doubling time is greatly increased.



Culture Conditions: Cells incubated at 37°C with 5% CO2.



Cryoprotectant Medium: 10% DMSO + 90% FCS



Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.



Additional Information:



Mutations:

- Homozygous deletion CDKN2A.

Medium:
Growth Condition:

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