Cell Line Description: Melanoma cell line. From metastatic site: Lymph node.



Also Known As:



Organism: Human (Homo sapiens)



Tissue: Skin



Growth Properties: Adherent



Morphology: Epithelial



Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS)



Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.



Subculturing Procedure:



Medium Renewal: MM537 requires growth medium to be changed 3 times each week.



Subcultivation Ratio: 1:4. When seeded at 2 x 106 cells/T75 flask, the cells will need to be passaged every 5 days. Split sub-confluent flasks (70-80% confluent). Flasks do not reach confluence. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cell line takes 2-3 days to recover from trypsinisation.



Culture Conditions: Cells incubated at 37°C with 5% CO2.



Cryoprotectant Medium: 10% DMSO + 90% FCS



Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.



Additional Information:



Mutations:



Homozygous Deletion CDKN2A
V600E BRAF

Medium:
Growth Condition:

BioVector NTCC Inc.
www.biovector.net