Description: NZM4 is a semi-adherent cell line derived from a 56-year-old male patient with metastatic melanoma (peritoneal effusion).



Description Key Words: Human metastatic melanoma



Also Known As: No other names



Organism: Human (Homo sapiens)



Tissue: Metastatic melanoma from ascites



Growth Properties: Adherent and floating viable cells



Doubling Time: 48 hours



Morphology: Epithelial



Growth Medium: This cell line was established by the depositor in: Alpha modified Minimum Essential Medium (MEM) supplemented with 5% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%O2, 5%CO2 and 90%N2.



At CellBank Australia it has been cultured in: Alpha modified Minimum Essential Medium (MEM) supplemented with 10% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%CO2.



Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.



Subculturing Procedure:



Medium Renewal: 2-3 times per week.



Subcultivation Ratio: 1:4 – 1:8, Seeding density 1.3 x104cells/cm2. Split subconfluent cultures(70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.



Culture Conditions: Incubate the culture at 37°C with 5% CO2.



Cryoprotectant Medium: 10% DMSO + 90% FCS



Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.



Additional Information:

Wild-type for NRAS, PIK3CA, CDKN2A/B
p53 mutant S241P
Resistant to temozolomide
MGMT expressed.

Medium:
Growth Condition:

BioVector NTCC Inc.
www.biovector.net