Description: SIRMu-1 is an immortalized rat Müller-1 cell line, monoclonal, derived from 2 sequential single cell/clonal isolations.
Note: Müller cells are a major type of glial cells in the retina of the eye.



Organism: Rat (Rattus norvegicus)



Strain: Sprague-Dawley



Tissue: Retina



Growth Properties: Adherent



Morphology: Epithelial-like



Growth Medium: EMEM + 20% Foetal Calf Serum (FCS) + 25mM Glucose + 2mM L-Glutamine



Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.



NOTE: This cell line may take up to 48hours to seed.



Subculturing Procedure:



Medium Renewal: 2-3 times per week



Subcultivation Ratio: 1:5 - 1:10, split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

At thaw, the cells should be seeded at a density of 2-3 x 104cells/cm2. After recovery from thaw (48-72 hours), they can be seeded at 0.5-1.0 x 104cells/cm2.

Flasks will require passaging twice weekly at a 1:10 seeding density.



Culture Conditions: Incubate the culture at 37°C with 5% CO2.



Cryoprotectant Medium: 10% DMSO + 90% FCS.



Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.



Additional Information:

The cells express the Müller and/or glial cells markers:

Glutamine Synthetase
S-100 Protein
Vimentin
Glial Fibrillary Acidic Protein (GFAP)
Gender: Male

Medium:
Growth Condition:

BioVector NTCC Inc.
www.biovector.net