pNIC-CTHF plasmid vector原核表達質粒載體

(Empty Backbone) SGC Empty backbone for bacterial expression

pET-28-a
(Search Vector Database)
Backbone manufacturerNovagen
Backbone size (bp)5330
Vector typeBacterial Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Growth instructionsFor plasmid propagation and cloning use any E. coli strain not expressing T7 RNA polymerase. For expression, use a strain that expresses T7 RNA polymerase, e.g. BL21(DE3).
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameNone
GenBank IDEF199844
Tag / Fusion Protein
TEV - His6 - Flag (C terminal on backbone)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteLigation-Independent cloning; cut with BfuAI (destroyed during cloning)
3′ cloning siteLigation-Independent cloning; cut with BfuAI (destroyed during cloning)
5′ sequencing primerT7F
3′ sequencing primerT7R

Primers for LIC cloning:

Upstream: add TTAAGAAGGAGATATACTATG to the 5’ end (ATG in-frame with the desired coding
sequence).

Downstream: add GATTGGAAGTAGAGGTTCTCTGC to 5’ end of downstream primer (no termination codon!)

Supplier來源:BioVector NTCC Inc.
TEL電話:+86-010-53513060
Website網址: http://www.biovector.net