pOET8.VE3桿狀病毒表達載體

pOET8.VE3 is a baculovirus transfer vector designed for high level expression of
foreign genes under the powerful AcMNPV polyhedrin gene (polh) promoter. Derived
from the pUC57 vector, it contains a vankyrin expression cassette, P-vank-1, which
encodes an anti-apoptotic protein to help delay cell death following virus infection. It
has a bacterial origin of replication and an ampicillin resistance gene for selection in
E. coli whilst the polh sequences have been replaced by a multiple cloning site (MCS)
containing unique restriction enzyme sites for insertion of the foreign gene in the
correct orientation (see circular map below). The pOET8.VE3 vector features an
additional Honey Bee Melittin (HBM) signal sequence to target protein secretion and
a C-terminal 6xHis-tag fusion sequence to facilitate protein purification. pOET8.VE3 is
compatible with any baculovirus expression system that utilizes homologous
recombination in insect cells.



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