lenticrispr v2-BSD plasmid vector慢病毒基因編輯質粒載體

Mammalian expression of Cas9 and sgRNA scaffold

Backbone size w/o insert (bp)10000
Total vector size (bp)14873
Modifications to backbonepuromycin N-acetyltransferase was replaced by blasticidin S deaminase
Vector typeMammalian Expression, Lentiviral, CRISPR
Selectable markersBlasticidin
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature30°C
Growth Strain(s)NEB Stable
Growth instructionsNo more than 16-hour culture.
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameBlasticidin S deaminase
MutationReplaced puromycin N-acetyltransferase on the original plasmid
PromoterEF-1α
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning siteSacII (not destroyed)
5′ sequencing primerTGCTGAAACAAGCCGGAGAT
3′ sequencing primerAATTAGCCCTTCCAGTCCCC, WPRE_R

Supplier來源:BioVector NTCC Inc.
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